The Immortal Life of Henrietta Lacks by Rebecca Skloot Read Free Book Online

tweezing, and pipetting.
    By the time Henrietta walked into Hopkins, Mary was handling most of the tissue samples that came through the door, and so far all samples from TeLinde’s patients had died.
    At that point, there were many obstacles to growing cells successfully. For starters, no one knew exactly what nutrients they needed to survive, or how best to supply them. Many researchers, including the Geys, had been trying for years to develop the perfect culture medium—the liquid used for feeding cells. The recipes for Gey Culture Medium evolved constantly as George and Margaret added and removed ingredients, searching for the perfect balance. But they all sounded like witches’ brews: the plasma of chickens, purée of calf fetuses, special salts, and blood from human umbilical cords. George had rigged a bell and cable from the window of his lab across a courtyard to the Hopkins maternity ward, so nurses could ring anytime ababy was born, and Margaret or Mary would run over and collect umbilical cord blood.
    The other ingredients weren’t so easy to come by: George visited local slaughterhouses at least once a week to collect cow fetuses and chicken blood. He’d drive there in his rusted-out old Chevy, its left fender flapping against the pavement, shooting sparks. Well before dawn, in a rundown wooden building with a sawdust floor and wide gaps in the walls, Gey would grab a screaming chicken by the legs, yank it upside down from its cage, and wrestle it to its back on a butcher block. He’d hold its feet in one hand and pin its neck motionless to the wood with his elbow. With his free hand, he’d squirt the bird’s chest with alcohol, and plunge a syringe needle into the chicken’s heart to draw blood. Then he’d stand the bird upright, saying, “Sorry, old fella,” and put it back in its cage. Every once in a while, when a chicken dropped dead from the stress, George took it home so Margaret could fry it for dinner.
    Like many procedures in their lab, the Gey Chicken Bleeding Technique was Margaret’s creation. She worked out the method step-by-step, taught it to George, and wrote detailed instructions for the many other researchers who wanted to learn it.
    Finding the perfect medium was an ongoing experiment, but the biggest problem facing cell culture was contamination. Bacteria and a host of other microorganisms could find their way into cultures from people’s unwashed hands, their breath, and dust particles floating through the air, and destroy them. But Margaret had been trained as a surgical nurse, which meant sterility was her specialty—it was key to preventing deadly infections in patients in the operating room. Many would later say that Margaret’s surgical training was the only reason the Gey lab was able to grow cells at all. Most culturists, like George, were biologists; they knew nothing about preventing contamination.
    Margaret taught George everything he knew about keeping cultures sterile, and she did the same with every technician, grad student, and scientist who came to work or study in the lab. She hired a local woman named Minnie whose sole job was washing the laboratoryglassware using the only product Margaret would allow: Gold Dust Twins soap. Margaret was so serious about that soap, when she heard a rumor that the company might go out of business, she bought an entire boxcar full of it.
    Margaret patrolled the lab, arms crossed, and leaned over Minnie’s shoulder as she worked, towering nearly a foot above her. If Margaret ever smiled, no one could have seen it through her ever-present surgical mask. She inspected all the glassware for spots or smudges, and when she found them—which was often—she’d scream, “MINNIE!” so loud that Mary cringed.
    Mary followed Margaret’s sterilizing rules meticulously to avoid her wrath. After finishing her lunch, and before touching Henrietta’s sample, Mary covered herself with a clean white gown, surgical cap, and mask, and then